step per mm Failure

Hi dears
I try to calculate my step_mm in this way:
StepsPerTurn = 400 //my stepper motors are 0.9 degree 
BeltDistance = 2.0mm // GT2 belt 
GroovesInPulley = 10 // FOR ME IS 10
Substepping = 16//for a 1/16 step needed
StepsPerMM = StepsPerTurnSubstepping/(BeltDistanceGroovesInPulley)
= 40016/(2.0*10) =320

it should be noted i use drv8825 stepper motor driver.
but when i add this number for step per mm my printer dosent work correctly.
what can i do???


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