I've my Raspberry PI 3 connected via wifi with a dedicated external wifi dongle, and i've enabled the "internal Access Point feature" if the external wifi go down.
Analyzing the server log I noticed that unfortunately due an unstable wifi link the internal "Access Point" feature is often activated, and when it happens the server stops sending data to the printer for a few seconds, even then it continues printing.
I noticed this "bug" because obviously the nozzle creates a random dot in my printed object.
When disabling this feature I've not had this problem, now the internal "Access Point" feature is always off and all work fine, without pause.
So Roland please check if there's something wrong when the Access Point go up or down, probably it freezes for an instant the communication on the USB port.