Nope i use G32 S2 command.
And i try with G32 S2 in start g-code and also do it's manually send G32 S2 then slice and print.
Effect is the same no compensation in Z axis.
But i think when you use G32 with S2 parament you do this once (or if you think your bed has moved). You don't need have this in g-code start.